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He tōrākenga o ngā wāhanga o te wai he take mātua kia whai hua ngā whakamātautau. I te whakamārama i ngā kāpae, i te whakakōkō i ngā rēhita PCR, i te whakamāmā i ngā tauira hei whakamātautau i te whānuitanga o te wai, he iti ngā hapa i ngā wāhanga o te wai e whakakōkō nei, engari he nui ngā whāinga o ānei i te whakamātautau katoa. He whakataukī, he tauira kāore i whakamahinga, he kore whakamātautau i ngā ture mai i te whakamāmā i ngā wāhanga o te wai. Ko ngā pūrākau pai ko ngā ara māmā hei whakamāhi. Ko ngā whakamāramatanga e whā nei hei āwhina kia whai hua te whakamāmā i ngā wāhanga o te wai i tōu rūma whakamātautau.
1. Whiriwhiri i te pipete me ngā kōpae e tika ana
Ko te tuatahi o ngā mahi pai i te whakamāmā i ngā wāhanga o te wai ko te whakamāhi i te rākau e tika ana mō te wāhanga. Mā te whakamāhi i tēnei pipete 1000 µL mō te wāhanga 10 µL, kāore he whakamātautau tika i ngā wāhanga o te wai, nā te mea kāore he whakamātautau i tōna rohe whakamātautau. Ko te tikanga: whakamāhi i tōu pipete i te 35 ki te 100% o tōna wāhanga tūmatua. Whakamāhi ngā pipete whakamāngai tūturu, i ngā rākau whakamāmā wai automati kia iti ake i te 1 µL.
Te whakamātautau i te tīpako tika he mea rite tonu te whakamātautau i te pipette tika. Ko ngā tīpako tātai kāore i tēnei wā e whai ana i te whakamātautau tika ki ngā pipette katoa, ā, ka hāngai ai ki ngā whakamātautau hau me ngā hapa o ngā wāhi. Me whakamātau koe i ngā tīpako e tohu ana te kaiwhakamahi pipette, i tātai ai hei tīpako e whakaaetia ana mō tō pipette. Ko ngā tīpako kōwhiri he whakamārama hei whakamārama i ngā whakamātautau aerohe, engari ka pā te whakamātautau o ngā tau o te tīpako; me whakamātau anō i ngā tīpako kia tātai ai i ngā wāhi e hāngai ana. Ko ngā tīpako iti te whakamātautau he pai rawa mō ngā wāhi pākoki, ā, ka whakamātau i te whakamātautau o ngā wāhi kia kore ai te whakamātautau o ngā wāhi ki ngā tīpako, me ngā wāhi pākoki kia tātai ai te whakamātautau o ngā tīpako. Ko te whakamātautau mua i te whakamātautau o ngā wāhi, i te whakamātautau i ngā wāhi 2–3 wā i mua i te whakamātautau, ka whakamātau i te hau i roto i te tīpako, ā, ka whakamātau i ngā whakamātautau o ngā wāhi pākoki.
2. Whakamātau i ngā āhua o te taiao
I ngā āhuatanga o te taiao e whai pānga ana ki te whakamahi i ngā wāhi rākau, ko te hōhā o te wāhi rākau te mea matua. Ko ngā wāhi rākau e whanawhana ana me ngā whakamāmā i ngā hōhā, ā, ko ngā pipette i whakamāmātia i te hōhā o te wāhi—i te nuinga o ngā wā, i te 20–25 °C. Ko te rerekētanga nui i waenganui i te hōhā o te whare pūtaiao me te hōhā o ngā tauira, o ngā rākau rānei, ka whakamāngai i ngā hāngā o ngā wāhi e tukuna ana. Me whakamātau koe i ngā tauira me ngā rākau kia noho i te hōhā o te whare i mua i tōu timata i te whakamāmā i ngā wāhi rākau. He tohua te whakamahinga o tēnei wāhi rākau i te wāhi rākau e whakamāmātia ana, he rākau rānei e whakamāmātia ana hei whakamāmā i ngā whakamāmā e pā ana ki te hōhā.
Ko te hāngaitanga hoki he mea matua. Ko ngā hāngaitanga iti he take o ngā ngāwhā o te whakamātautau i ngā kaiwhakamātautau kōpū; he iti ake te rahi o ngā kaiwhakamātautau i tukuna. Ko ngā hāngaitanga mātua he take o te whakamātautau o te wai i roto i te pipette, e whakamāngai ana i te whakamātautau o te wāhi o te hau. Me mahi koe i ngā kaiwhakamātautau kōpū i te wā pūmau, ā, whakaarohia te whakamāhi i ngā pipette whakamātautau tūturu, i ngā kaiwhakamātautau auomatua hoki e whakamāmā ana i te nekehanga o te hau mō ēnei momo kaiwhakamātautau. Ko ngā hau mai i ngā matātoko kūwae, i ngā whakamātautau hau, i ngā mahi i roto i te whare whakamātautau he take o ngā whakamātautau korekore i te whakamātautau o te whakamātautau o te tāwhai o te pipette, o te tau o te wai.
3. Whakamāhi i ngā tikanga whakamātautau tika
Ahakoa te pipette e whakamāhua ana i te whakamāhua tika rawa, he rerekē ngā hua mēnā kāore he whakamāhiatanga tika. Whakamārama i ngā tikanga e tātari ana. Whakamāhinga i te whakamāhiatanga o te whakamāhiatanga o te tip mā te whakamāhinga i te wai me te whakamāhinga i te wai e toru ki te rima wā i mua i te whakamāhinga mō te whakakī, hei whakapai ake i te hukapapa i roto i te pipette me te whakamāmā i ngā ngāwari o te whakamāhinga. Tūhono i te tip o te pipette i raro i te whakamāhinga o te 2-4 mm mō ngā wāhanga iti, i raro i te whakamāhinga o te 4-6 mm mō ngā wāhanga nui i te wā o te whakamāhinga. Kaua e whakamāhinga i te tip i raro tonu, ā, ka pāngia te wai mātātoko ki waho o te tip.
Me pēhi āta, me te pūmau i te pūwiri kia pupuhi ai te wai. Me karo te tuku tere i te pūngāwerewere, ka puta ai ngā ngū, ngā kōhū hau rānei, ā, ka taea e te tuku puhoi te whakaputa i te tauira ki roto i te ipu pipette. Me mutu te whakamutu mō te hēkona kotahi i muri i te whakaoho kia taea ai te whakauru i te rōrahi katoa ki te pito. Whakanohoia te pito ki te kokonga 30-45 nekehanga ki te pakitara o te ipu kaiwhiwhi i te wa e tuku ana. Pirihia te pūhāhā ki te tūnga tuatahi, mutu kotahi hēkona, ka pēhi ki te tūnga tuarua mō te tuku katoa o te wai. Me whakaaro ki te whakamahi i te pipetting whakamuri hei whakapai ake i te tika ki nga rōrahi iti rawa, ki nga tauira tino kakara rānei.
4. Te whakaū i te whare. Whakamahia te Pūmanawa i te wā e taea ai
Ko te rerekētanga o te kaiwhakahaere e hāngai ana ki te pipetting ā-ringa - ngā rerekētanga i roto i te tikanga, te ngenge, te whakapohehe rānei - ka taea te tāpiri ki ngā rerekētanga i roto i ō hua. Ka tukuna e ngā kaiwhakarato wai aunoa te rōrahi tika i ia wā. Kia whakahaeretia tētahi aratuka, ka whakahoutia e tētahi kaiwhakahaere wai mō ngā mano o ngā tautohe, kāore he rerekētanga ahakoa ko wai e tīmata ana i te rere.
Ko ngā kāpae whakamātautau he pai hoki i te whakamārama i te pātata o te whakamātautau i te rōrahi o ngā pipette, me ngā hāngā o te whakamātautau me ngā hāngā o te whakamātautau; ā, ko ēnei āhua e rua he take matua mō ngā rōrahi o te whakamātautau i ngā wāhanga. Ko ngā whakamātautau tāwhai he tino pai mō ngā whakamātautau, he iti ake ngā whāinga o ngā hāngā o te tangata i ēnei hāngā roa me ngā hāngā e whakamātau ana. Ko ngā kāpae whakamātautau me ngā kākāi wai o Hangzhou Zhongwang Technology he whakamātau pai mō te whakamārama i te whakamārama o te whakamārama me ngā whakamārama o te whakamārama mō ngā whare whakamātautau o te whakamārama, me ngā wāhi katoa i tēnei wāhanga o te wai e whakamārama ana i ngā whakamārama.
Whakarāpopoto
Ko te whakamāhua pai i ngā wāhi rākau he mea e te whiriwhiri tātai i ngā pipette me ngā tip, te whakahaere i ngā āhuatanga o te taiao, te whakamātau i ngā tikanga pai, me te whakamātau i ngā rākau automata i ngā wā e tika ana. Ko ia wāhi e āki ana i te iti o te whāinga o te whakamāhua, ā, e whakapai ana i te whakamāmā o ngā whakamāhua i tāu rūma pūtaiao. Ko Hangzhou Zhongwang Technology Co., Ltd. he rākau automata, ngā rākau whakamāhua wāhi, ngā rākau whakamāhua kōpū, ngā rākau whakamāhua me ngā rākau whakamāhua hōu, ngā rākau whakamāhua mākū, ngā rākau whakamāhua āhua, ngā rākau whakamāhua vaku, ngā rākau whakamāhua capillary viscometer, me ngā rākau whakamāhua. Whakapā mai i tēnei wā kia kite ai he aha tātou e whai ai mōu.